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Effects of Smoking on the Ex Vivo Cytokine Production in Periodontitis by G. L. Torres de Heens; R. Kikkert; L. A. Aarden; U. Van Der Velden; B. G. Loos is a Dentistry article available to read on EtoBox.

What is Effects of Smoking on the Ex Vivo Cytokine Production in Periodontitis about?

**Background and Objective:** Smoking is associated with increased severity of periodontitis. The underlying mechanisms of this phenomenon are not well understood. The purpose of the present study was to compare the monocyte‐derived T cell directing (Th1/Th2) response and pro‐inflammatory cytokine production in __ex vivo__ whole blood cell cultures (WBCC) of smoking and non‐smoking chronic periodontitis patients. **Material and Methods:** Venous blood was collected from 29 periodontitis patients (18 non‐smokers and 11 smokers) receiving supportive periodontal treatment, and diluted 10‐fold for WBCC. The WBCC were stimulated for 18 h with __Neisseria meningitidis__ lipo‐oligosaccharide (LOS) or__Porphyromonas gingivalis__ sonic extract (Pg‐SE). The production of the T cell directing cytokines interleukin (IL)‐12 p40 and IL‐10, as well as the pro‐inflammatory cytokines IL‐1β, IL‐6 and IL‐8, was measured in the culture supernatants. **Results:** After LOS stimulation of WBCC, smokers showed a lower IL‐12 p40/IL‐10 ratio than non‐smokers (__P <__ 0.05). Interleukin‐1β production was significantly lower in smokers compared with non‐smokers after stimulation with either LOS or Pg‐SE (__P

Who reads Effects of Smoking on the Ex Vivo Cytokine Production in Periodontitis?

Author

G. L. Torres de Heens; R. Kikkert; L. A. Aarden; U. Van Der Velden; B. G. Loos

Publisher

Wiley

Published

2009

Language

EN

Field

Dentistry (Health Sciences)