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Role of group A Streptococcus HtrA in the maturation of SpeB protease by Jason N. Cole; John A. Aquilina; Peter G. Hains; Anna Henningham; Kadaba S. Sriprakash; Michael G. Caparon; Victor Nizet; Malak Kotb; Stuart J. Cordwell; Steven P. Djordjevic; Mark J. Walker is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract The serine protease high‐temperature requirement A (HtrA) (DegP) of the human pathogen __Streptococcus pyogenes__ (group A __Streptococcus__; GAS) is localized to the ExPortal secretory microdomain and is reportedly essential for the maturation of cysteine protease streptococcal pyrogenic exotoxin B (SpeB). Here, we utilize HSC5 (M5 serotype) and the in‐frame isogenic mutant HSC5Δ__htrA__ to determine whether HtrA contributes to the maturation of other GAS virulence determinants. Mutanolysin cell wall extracts and secreted proteins were arrayed by 2‐DE and identified by MALDI‐TOF PMF analysis. HSC5Δ__htrA__ had elevated levels of cell wall‐associated M protein, whilst the supernatant had higher concentrations of M protein fragments and a reduced amount of mature SpeB protease, compared to wild‐type (WT). Western blot analysis and protease assays revealed a delay in the maturation of SpeB in the HSC5Δ__htrA__ supernatant. HtrA was unable to directly process SpeB zymogen (proSpeB) to the active form __in vitro__. We therefore conclude that HtrA plays an indirect role in the maturation of cysteine protease SpeB.
Who reads Role of group A Streptococcus HtrA in the maturation of SpeB protease?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jason N. Cole; John A. Aquilina; Peter G. Hains; Anna Henningham; Kadaba S. Sriprakash; Michael G. Caparon; Victor Nizet; Malak Kotb; Stuart J. Cordwell; Steven P. Djordjevic; Mark J. Walker
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)