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Contribution of SecDF to Staphylococcus aureus resistance and expression of virulence factors by Chantal Quiblier; Annelies S Zinkernagel; Reto A Schuepbach; Brigitte Berger-Bächi; Maria M Senn is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Contribution of SecDF to Staphylococcus aureus resistance and expression of virulence factors about?
## Background SecDF is an accessory factor of the conserved Sec protein translocation machinery and belongs to the resistance-nodulation-cell division (RND) family of multidrug exporters. SecDF has been shown in __Escherichia coli__ and __Bacillus subtilis__ to be involved in the export of proteins. RND proteins can mediate resistance against various substances and might be of relevance in antimicrobial therapy. The role of RND proteins in __Staphylococcus aureus__ has not yet been determined. ## Results Markerless deletion mutants were constructed to analyze the impact of the so far uncharacterized RND proteins in __S. aureus__. While the lack of Sa2056 and Sa2339 caused no phenotype regarding growth and resistance, the __secDF__ mutant resulted in a pleiotropic phenotype. The __secDF__ mutant was cold sensitive, but grew normally in rich medium at 37°C. Resistance to beta-lactams, glycopeptides and the RND substrates acriflavine, ethidium bromide and sodium dodecyl sulfate was reduced. The __secDF__ mutant showed an aberrant cell separation and increased spontaneous and Triton X-100 induced autolysis, although the amounts of penicillin-binding proteins in the membrane were unchan
Who reads Contribution of SecDF to Staphylococcus aureus resistance and expression of virulence factors?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Chantal Quiblier; Annelies S Zinkernagel; Reto A Schuepbach; Brigitte Berger-Bächi; Maria M Senn
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2180)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)