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The Inhibition of Prions Through Blocking Prion Conversion by Permanently Charged Branched Polyamines of Low Cytotoxicity by Yong-beom Lim; Charles E. Mays; Younghwan Kim; William B. Titlow; Chongsuk Ryou is a Engineering article available to read on EtoBox.

What is The Inhibition of Prions Through Blocking Prion Conversion by Permanently Charged Branched Polyamines of Low Cytotoxicity about?

Branched polyamines are effective in inhibiting prions in a cationic surface charge density dependent manner. However, toxicity associated with branched polyamines, in general, often hampers the successful application of the compounds to treat prion diseases. Here, we report that constitutively maintained cationic properties in branched polyamines reduced the intrinsic toxicity of the compounds while retaining the anti-prion activities. In prion-infected neuroblastoma cells, quaternization of amines in polyethyleneimine (PEI) and polyamidoamine (PAMAM) dendrimers markedly increased the nontoxic concentration ranges of the compounds and still supported, albeit reduced, an appreciable level of anti-prion activity in clearing prions from the infected cells. Furthermore, quaternized PEI was able to degrade prions at acidic pH conditions and inhibit the in vitro prion propagation facilitated by conversion of the normal prion protein isoform to its misfolded counterpart, although such activities were decreased by quaternization. Quaternized PAMAM was least effective in degrading prions but efficiently inhibited prion conversion with the same efficacy as unmodified PAMAM. Our results sugg

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Author

Yong-beom Lim; Charles E. Mays; Younghwan Kim; William B. Titlow; Chongsuk Ryou

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)

Published

2010

Language

EN

Field

Engineering (Physical Sciences)