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Can I read Loss of Heterozygosity at the NAD(P)H:Quinone Oxidoreductase Locus Associated with Increased Resistance against Mitomycin C in a Human Bladder Carcinoma Cell Line on EtoBox?
Loss of Heterozygosity at the NAD(P)H:Quinone Oxidoreductase Locus Associated with Increased Resistance against Mitomycin C in a Human Bladder Carcinoma Cell Line by Eickelmann, Peter; Schulz, Wolfgang A.; Rohde, Detlef; Schmitz-Dräger, Bernd; Sies, Helmut is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Loss of Heterozygosity at the NAD(P)H:Quinone Oxidoreductase Locus Associated with Increased Resistance against Mitomycin C in a Human Bladder Carcinoma Cell Line about?
The human bladder carcinoma cell line RT112 and the mitomycin C-resistant cell line RT112MMC, derived from RT112 cells, were examined for their expression of NAD(P)H:quinone Oxidoreductase (NQOR) and glutathione S-transferases (GSTs). RT112 cells were 40-fold more sensitive towards mitomycin C than RT112MMC cells. The NQOR mRNA level in RT112MMC cells was decreased to 15% as compared to RT112 cells. NQOR enzyme activity was 391 ± 140 mU/mg protein in RT112 cells, whereas NQOR activity in RT112MMC cells was not measurable. As shown by a fast PCR-based assay and DNA-sequencing, the cell line RT112 is heterozygous, whereas RT112MMC is homozygousfora null alleleof the NQOR gene without enzymatic activity. Accordingly, both wild-type and null allele mRNAs were present in RT112 cells, whereas only null allele mRNA was found in RT112MMC. The lack of NQOR enzyme activity in RT112MMC cells was thus associated with loss of heterozygosity at the NQOR locus. By a PCR-RFLP assay, three kidney carcinoma patients without measurable NQOR enzyme activity were shown to be homozygous for the null allele. The PCR assay described here is useful for examination of large numbers of samples. The relative
Who reads Loss of Heterozygosity at the NAD(P)H:Quinone Oxidoreductase Locus Associated with Increased Resistance against Mitomycin C in a Human Bladder Carcinoma Cell Line?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Eickelmann, Peter; Schulz, Wolfgang A.; Rohde, Detlef; Schmitz-Dräger, Bernd; Sies, Helmut
- Publisher
- Walter de Gruyter GmbH (ISSN 0177-3593)
- Published
- 1994
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)