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Antibody targeting of a specific region of Pfs47 blocks Plasmodium falciparum malaria transmission by Canepa, Gaspar E.; Molina-Cruz, Alvaro; Yenkoidiok-Douti, Lampouguin; Calvo, Eric; Williams, Adeline E.; Burkhardt, Martin; Peng, Fangni; Narum, David; Boulanger, Martin J.; Valenzuela, Jesus G.; Barillas-Mury, Carolina is a Medicine article available to read on EtoBox.
What is Antibody targeting of a specific region of Pfs47 blocks Plasmodium falciparum malaria transmission about?
Transmission-blocking vaccines are based on eliciting antibody responses in the vertebrate host that disrupt parasite development in the mosquito vector and prevent malaria transmission. The surface protein Pfs47 is present in __Plasmodium falciparum__ gametocytes and female gametes. The potential of Pfs47 as a vaccine target was evaluated. Soluble full-length recombinant protein, consisting of three domains, was expressed in __E. coli__ as a thioredoxin fusion (T-Pfs47). The protein was immunogenic, and polyclonal and monoclonal antibodies (mAb) were obtained, but they did not confer transmission blocking activity (TBA). All fourteen mAb targeted either domains 1 or 3, but not domain 2 (D2), and immune reactivity to D2 was also very low in polyclonal mouse IgG after T-Pfs47 immunization. Disruption of the predicted disulfide bond in D2, by replacing cysteines for alanines (C230A and C260A), allowed expression of recombinant D2 protein in __E. coli__. A combination of mAbs targeting D2, and deletion proteins from this domain, allowed us to map a central 52 amino acid (aa) region where antibody binding confers strong TBA (78-99%). This 52 aa antigen is immunogenic and well conserved
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- Author
- Canepa, Gaspar E.; Molina-Cruz, Alvaro; Yenkoidiok-Douti, Lampouguin; Calvo, Eric; Williams, Adeline E.; Burkhardt, Martin; Peng, Fangni; Narum, David; Boulanger, Martin J.; Valenzuela, Jesus G.; Barillas-Mury, Carolina
- Publisher
- Springer Science and Business Media LLC; Nature Publishing Group; London: Springer Nature, 2016- (ISSN 2059-0105)
- Published
- 2018
- Language
- EN
- Field
- Medicine (Health Sciences)