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Differential expression and cellular distribution of γ‐tubulin and βIII‐tubulin in medulloblastomas and human medulloblastoma cell lines by Valentina Caracciolo; Luca D'agostino; Eduarda Dráberová; Vladimíra Sládková; Catena Crozier‐Fitzgerald; Dimitri P. Agamanolis; Jean‐Pierre de Chadarévian; Agustin Legido; Antonio Giordano; Pavel Dráber; Christos D. Katsetos is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Differential expression and cellular distribution of γ‐tubulin and βIII‐tubulin in medulloblastomas and human medulloblastoma cell lines about?
## Abstract In previous studies, we have shown overexpression and ectopic subcellular distribution of γ‐tubulin and βIII‐tubulin in human glioblastomas and glioblastoma cell lines (Katsetos et al., 2006, J Neuropathol Exp Neurol 65:455–467; Katsetos et al., 2007, Neurochem Res 32:1387–1398). Here we determined the expression of γ‐tubulin in surgically excised medulloblastomas (n = 20) and in the human medulloblastoma cell lines D283 Med and DAOY. In clinical tissue samples, the immunohistochemical distribution of γ‐tubulin labeling was pervasive and inversely related to neuritogenesis. Overexpression of γ‐tubulin was widespread in poorly differentiated, proliferating tumor cells but was significantly diminished in quiescent differentiating tumor cells undergoing neuritogenesis, highlighted by βIII‐tubulin immunolabeling. By quantitative real‐time PCR, γ‐tubulin transcripts for TUBG1, TUBG2, and TUBB3 genes were detected in both cell lines but expression was less prominent when compared with the human glioblastoma cell lines. Immunoblotting revealed comparable amounts of γ‐tubulin and βIII‐tubulin in different phases of cell cycle; however, a larger amount of γ‐tubulin was detected
Who reads Differential expression and cellular distribution of γ‐tubulin and βIII‐tubulin in medulloblastomas and human medulloblastoma cell lines?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Valentina Caracciolo; Luca D'agostino; Eduarda Dráberová; Vladimíra Sládková; Catena Crozier‐Fitzgerald; Dimitri P. Agamanolis; Jean‐Pierre de Chadarévian; Agustin Legido; Antonio Giordano; Pavel Dráber; Christos D. Katsetos
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)