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Ursolic acid loaded β-cyclodextrin/folic acid/Fe3O4 nanocomplex for drug delivery to tumor cells 1 1 Introduction 1 2 Materials and methods 2 2.1 Materials 2 2.2 Synthesis of UA-β-CD-Fe3O4-FA-NPs 2 2.3 Characterization of the nanocomplex 3 2.4 UA loading capacity in nanocomplex 3 2.5 In-vitro drug release studies 3 2.6 Drug release kinetics 3 2.7 Cell culture and MTT assay 3 2.8 Assessment of apoptosis by flow cytometry 3 2.9 In vivo study 4 2.9.1 Animal models 4 2.9.2 Antitumor effectiveness 4 2.10 Concentration of iron in tissues 4 2.11 Hematology and cytokine measurements 4 2.12 Histopathological analysis 4 2.13 Immunohistochemistry (IHC) 4 2.14 Statistical analysis 5 3 Results 5 3.1 Characterization of the nanocomplex 5 3.2 Size distribution and surface charge 6 3.3 UA loading capacity and in vitro release 7 3.4 Drug release kinetics 7 3.5 MTT assay 7 3.6 Apoptosis in MCF-7 cells induced by UA-β-CD/FA/Fe3O4 NPs 7 3.7 Antitumor effectiveness 8 3.8 Iron concentration in tumor and liver 8 3.9 Hematological parameters 9 3.10 Histopathology 9 4 Discussion 9 5 Conclusion 11 CRediT authorship contribution statement 12 Declaration of competing interest 12 References 12

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Language

UND

Category

nonfiction