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TA Cloning: A Simple Guide by Deepika Dhanasekar is a document available to read on EtoBox.

What is TA Cloning: A Simple Guide about?

TA cloning is a method for cloning DNA fragments with A overhangs into vectors with T overhangs, developed by TA Halton in 1990. The process involves creating the insert and vector, followed by ligation and transformation, and offers advantages such as higher efficiency and simplicity compared to traditional methods. However, it has limitations including non-directional cloning and the cost of kits.

Author

Deepika Dhanasekar

Language

EN