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E. Coli Lactate Biosensor for Cell Cultures by Sivapriya Chandran is a document available to read on EtoBox.
The document describes the development of a whole-cell E. coli lactate biosensor to monitor lactate accumulation in mammalian cell cultures during biopharmaceutical production. The biosensor was constructed using two genetic variations of the natural E. coli llprd lactate operon. Experiments showed the biosensor can accurately determine lactate concentrations in samples from conditions relevant to the biopharmaceutical industry, with a lower limit of detection than standard analytical methods. The biosensor
- Author
- Sivapriya Chandran
- Language
- EN