Can I read Functional Identification of Px-fringe and Px-engrailed Genes under Heat Stress in Chlorpyrifos-Resistant and -Susceptible Plutela xylostella (Lepidoptera: Plutellidae) on EtoBox?

Functional Identification of Px-fringe and Px-engrailed Genes under Heat Stress in Chlorpyrifos-Resistant and -Susceptible Plutela xylostella (Lepidoptera: Plutellidae) by Wang, Yu; Wang, Jingnan; Xia, Xiaofeng; Wu, Gang is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Functional Identification of Px-fringe and Px-engrailed Genes under Heat Stress in Chlorpyrifos-Resistant and -Susceptible Plutela xylostella (Lepidoptera: Plutellidae) about?

In our previous research, the fitness cost of resistance of the diamondback moth (DBM), __Plutella xylostella__ found in insecticide-resistant DBM (Rc-DBM) under heat stress was based on heavier damage to wing veins when compared to insecticide-susceptible DBM (Sm-DBM). To investigate the molecular mechanism of the damage to the veins between Rc- and Sm-DBM, the full-length sequences of two related genes involved in the development of wing veins, f__ringe__ (__Px__-__fng__) and __engrailed__ (__Px__-__en__) of DBM were cloned, and the mRNA expressions of both __Px-fng__ and __Px-en__ were studied. The __Px-fng__ and __Px-en__ cDNA contained 1038 bp and 1152 bp of open reading frames (ORFs), respectively, which encoded a putative protein comprising 345 and 383 amino acids with a calculated molecular weight of 39.59 kDa and 42.69 kDa. Significantly down regulated expressions of __Px-fng__ and __Px-en__ under heat stress were found in pupae and adults of Rc-DBM compared to Sm-DBM, and a result of higher damage to wing veins in Rc-DBM under heat stress. Based on RNAi experiments, significant inhibitions on expressions of __Px-fng__ and __Px-en__ in both Sm-DBM and Rc-DBM were found whe

Who reads Functional Identification of Px-fringe and Px-engrailed Genes under Heat Stress in Chlorpyrifos-Resistant and -Susceptible Plutela xylostella (Lepidoptera: Plutellidae)?

Author

Wang, Yu; Wang, Jingnan; Xia, Xiaofeng; Wu, Gang

Publisher

MDPI AG; Multidisciplinary Digital Publishing Institute (MDPI); Basel: MDPI AG, 2010- (ISSN 2075-4450)

Published

2020

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)