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Directly Observed Hydrogen Bonds at Calcium-binding-sites of Calmodulin in Solution Relate to Affinity of the Calcium-binding by Nenad Juranić; Elena Atanasova; Slobodan Macura; Franklyn G. Prendergast is a Chemistry article available to read on EtoBox.

What is Directly Observed Hydrogen Bonds at Calcium-binding-sites of Calmodulin in Solution Relate to Affinity of the Calcium-binding about?

Molecular tuning to calcium-binding in the EF-hand motif of holo-calmodulin was studied in solution by NMR (h3)J(NC') H-bond couplings. In the N-terminus lobe of holo-calmodulin, the glutamate crucial for Ca(2+) coordination has network of H-bonds weaker than inferred from the X-ray crystal structure. This glutamate at position 12 appears shifted away from the Ca(2+) preferred coordination, which can explain the lower affinity of the calcium-binding to the N-terminus with respect to C-terminus EF hands.

Who reads Directly Observed Hydrogen Bonds at Calcium-binding-sites of Calmodulin in Solution Relate to Affinity of the Calcium-binding?

Author

Nenad Juranić; Elena Atanasova; Slobodan Macura; Franklyn G. Prendergast

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0162-0134)

Published

2009

Language

EN

Field

Chemistry (Physical Sciences)