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Can I read Sex, Fiber-type, and Age Dependent in Vitro Proliferation of Mouse Muscle Satellite Cells on EtoBox?
Sex, Fiber-type, and Age Dependent in Vitro Proliferation of Mouse Muscle Satellite Cells by Raquel Manzano; Janne M. Toivonen; Ana C. Calvo; Francisco J. Miana-Mena; Pilar Zaragoza; María J. Muñoz; Didier Montarras; Rosario Osta is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Sex, Fiber-type, and Age Dependent in Vitro Proliferation of Mouse Muscle Satellite Cells about?
During postnatal growth and after muscle injury, satellite cells proliferate and differentiate into myotubes to form and repair musculature. Comparison of studies on satellite cell proliferation and differentiation characteristics is confounded by the heterogeneity of the experimental conditions used. To examine the influence of sex, age, and fiber-type origin on in vitro properties of satellite cells derived from postnatal muscles, fast extensor digitorum longus (EDL) and slow soleus (SOL) muscles were extracted from male and female mice of 1 week to 3 months of age. Myoblast proliferation and myogenic regulatory factor (MRF) expression was measured from cultures of freshly isolated satellite cells. Higher proliferation rate and elevated Myod1 expression was found in male EDL and SOL derived cells compared with females at age of 40, 60, and 120 days, whereas inverse tendency for cell proliferation was apparent in EDL of juvenile (7-day-old) pups. Myogenin and Mrf4 transcripts were generally elevated in males of 40 and 60 days of age and in female EDL of juveniles. However, these differentiation markers did not significantly correlate with proliferation rate at all ages. Pax7, whos
Who reads Sex, Fiber-type, and Age Dependent in Vitro Proliferation of Mouse Muscle Satellite Cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Raquel Manzano; Janne M. Toivonen; Ana C. Calvo; Francisco J. Miana-Mena; Pilar Zaragoza; María J. Muñoz; Didier Montarras; Rosario Osta
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)