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LncRNA NKILA knockdown promotes cell viability and represses cell apoptosis, autophagy and inflammation in lipopolysaccharide-induced sepsis model by regulating miR-140-5p/CLDN2 axis by Dan Han; Rong Fang; Rong Shi; Yuanyuan Jin; Qian Wang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is LncRNA NKILA knockdown promotes cell viability and represses cell apoptosis, autophagy and inflammation in lipopolysaccharide-induced sepsis model by regulating miR-140-5p/CLDN2 axis about?

## Background: Long non-coding rnas (lncrnas) play vital roles in human diseases, including sepsis-induced acute kidney injury (aki). here, we aimed to investigate the functions of lncrna nkila in sepsis-engendered aki. ## Methods: Hk2 cells stimulated with lps were used to mimic sepsis-induced aki in vitro. qrt-pcr was conducted for lncrna nkila and mir-140-5p levels. cell counting kit-8 (cck-8) assay and flow cytometry analysis were employed to analyze cell viability and apoptosis. western blot assay was utilized to measured protein levels. elisa kits were used to examine the concentrations of il-6, il-1β and tnf-α. dual-luciferase reporter assay was utilized to analyze the relationships among lncrna nkila, mir-140-5p and claudin 2 (cldn2). ## Results: Lps restrained hk2 cell viability and accelerated cell apoptosis and autophagy. lncrna nkila was increased in lps-treated hk2 cells. lncrna nkila silencing reversed the promotional influence of lps on cell progression in hk2 cells. mir-140-5p inhibition ameliorated lncrna nkila knockdown-mediated cell injury in lps-mediated hk2 cells. cldn2 was the target of mir-140-5p. mir-140-5p elevation promoted cell viability and suppressed ce

Who reads LncRNA NKILA knockdown promotes cell viability and represses cell apoptosis, autophagy and inflammation in lipopolysaccharide-induced sepsis model by regulating miR-140-5p/CLDN2 axis?

Author

Dan Han; Rong Fang; Rong Shi; Yuanyuan Jin; Qian Wang

Publisher

Elsevier BV

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)