About this Engineering article
Synergic effects of crypt-like topography and ECM proteins on intestinal cell behavior in collagen based membranes by Lin Wang; Shashi K. Murthy; Gilda A. Barabino; Rebecca L. Carrier is a Engineering article available to read on EtoBox.
The basement membrane of small intestinal epithelium possesses complex topography at multiple scales ranging from the mesoscale to nanoscale. Specifically, intestinal crypt-villus units are comprised of hundred-micron-scale well-like invaginations and finger-like projections; intestinal cell phenotype is related to location on this crypt-villus unit. A biomimetic intestinal cell culture system composed of type I collagen based permeable cell culture membranes incorporating both micron-scale intestinal crypt-like topography and nanometer scale topography was fabricated. Membranes were pre-incubated with either laminin (Ln) or fibronectin (Fn), inoculated with intestinal epithelial Caco-2 cells and cultured for 1-21 days to study the relative significance of influence of crypt-like topography and biomimetic substrate chemistry on cell phenotype. Crypt-like topography inhibited Caco-2 differentiation during early culture, as evidenced by slower cell spreading and lower brush border enzyme activity. For example, alanine aminopeptidase activity was lower on Ln-coated patterned collagen ( approximately 3.4+/-0.24mU/mg) compared to flat collagen (10.84+/-0.55mU/mg) at day 7. Caco-2 cultur
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Lin Wang; Shashi K. Murthy; Gilda A. Barabino; Rebecca L. Carrier
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)
- Published
- 2010
- Language
- EN
- Field
- Engineering (Physical Sciences)