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microRNA-222-Mediated VHL Downregulation Facilitates Retinoblastoma Chemoresistance by Increasing HIF1α Expression by Chunzhi Li; Jun Zhao; Weiying Sun is a Medicine article available to read on EtoBox.

What is microRNA-222-Mediated VHL Downregulation Facilitates Retinoblastoma Chemoresistance by Increasing HIF1α Expression about?

## Purpose Retinoblastoma (RB) is the most common primary intraocular tumor in children. Chemoresistance is the major obstacle for treatment of these tumors. This study aims to determine whether or not downregulating microRNA-222 (miR-222) could serve as a potential therapeutic target for preventing chemoresistance in RB treatment. ## Methods Differentially expressed miR-222 in RB samples and its downstream target __genes__ were predicted using bioinformatics methods. The expression of miR-222 was altered by mimic or inhibitor to examine its role in RB cell in response to the chemotherapeutic agent vincristine (VCR). Further bioinformatic analysis predicted involvement of the stability of hypoxia-inducible factor 1α (HIF1α) protein in regulation of the von Hippel–Lindau (VHL) tumor suppressor, followed by characterization of the effect of VHL on the ubiquitin–proteasome degradation of HIF1α. Next, VHL or HIF1α was overexpressed to determine their effects on RB cell activities after VCR treatment. In vivo assays were performed on nude mice to further verify the in vitro results. ## Results miR-222 is highly expressed in RB tissues and cells and was found to facilitate resistance of

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Author

Chunzhi Li; Jun Zhao; Weiying Sun

Publisher

Association for Research in Vision and Ophthalmology (ARVO)

Published

2020

Language

EN

Field

Medicine (Health Sciences)