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Hepatocytes May Produce Laminin in Fibrotic Liver and in Primary Culture by Bruno Clément; Pierre-Yves Rescan; Georges Baffet; Olivier Loréal; Dominique Lehry; Jean-Pierre Campion; André Guillouzo is a Medicine article available to read on EtoBox.
What is Hepatocytes May Produce Laminin in Fibrotic Liver and in Primary Culture about?
Previous studies have shown that laminin is present in basement membranes in normal liver but failed to identify cellular sources. We have investigated the extracellular and intracellular distribution of laminin in normal rat and human liver, in fibrotic human liver and in primary hepatocyte cultures from both species by light and electron microscopy using the indirect immunoperoxidase technique. In normal liver from both species, antibodies to laminin strongly stained basement membranes and formed discontinuous discrete deposits in the wall of the sinusoid. Vascular endothelial and bile duct cells as well as fat-storing cells and sinusoidal endothelial cells strongly stained for this glycoprotein while hepatocytes were negative. In fibrotic human liver, increased amounts of extracellular laminin were usually found. Continuous deposition in the space of Disse was observed in some cases. In addition to fat-storing cells and endothelial cells, hepatocytes were also sometimes positive. Normal rat and human hepatocytes synthesize and secrete laminin in conventional culture, but it remains soluble in the medium. By contrast, in coculture with another rat liver cell type, laminin accumul
Who reads Hepatocytes May Produce Laminin in Fibrotic Liver and in Primary Culture?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Bruno Clément; Pierre-Yves Rescan; Georges Baffet; Olivier Loréal; Dominique Lehry; Jean-Pierre Campion; André Guillouzo
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Elsevier BV; Wiley; Research Square (ISSN 0270-9139)
- Published
- 1988
- Language
- EN
- Field
- Medicine (Health Sciences)