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Proteomic identification of brain proteins that interact with dynein light chain LC8 by Inmaculada Navarro-Lérida; Mónica Martínez Moreno; Fernando Roncal; Francisco Gavilanes; Juan Pablo Albar; Ignacio Rodríguez-Crespo is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Proteomic identification of brain proteins that interact with dynein light chain LC8 about?
## Abstract Cytoplasmic dynein is a large minus end‐directed microtubule motor that translocates cargos towards the minus end of microtubules. Light chain 8 of the dynein machinery (LC8) has been reported to interact with a large variety of proteins that possess K/RSTQT or GIQVD motifs in their sequence, hence permitting their transport in a retrograde manner. Yeast two‐hybrid analysis has revealed that in brain, LC8 associates directly with several proteins such as neuronal nitric oxide synthase, guanylate kinase domain‐associated protein and gephyrin. In this work, we report the identification of over 40 polypeptides, by means of a proteomic approach, that interact with LC8 either directly or indirectly. Many of the neuronal proteins that we identified cluster at the post‐synaptic terminal, and some of them such as phosphofructokinase, lactate dehydrogenase or aldolase are directly involved in glutamate metabolism. Other pool of proteins identified displayed the LC8 consensus binding motif. Finally, recombinant LC8 was produced and a library of overlapping dodecapeptides (pepscan) was employed to map the LC8 binding site of some of the proteins that were previously identified usi
Who reads Proteomic identification of brain proteins that interact with dynein light chain LC8?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Inmaculada Navarro-Lérida; Mónica Martínez Moreno; Fernando Roncal; Francisco Gavilanes; Juan Pablo Albar; Ignacio Rodríguez-Crespo
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2004
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)