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This study investigates the extraction of bromelain from pineapple peel using reverse micelles, highlighting the advantages of the gemini surfactant C12-8-C12₂Br over the monomeric surfactant DTAB. Under optimized conditions, the gemini surfactant achieved a 163% activity recovery and a purification fold of 3.3, while DTAB resulted in 95% recovery and a purification fold of 1.7. The findings suggest that the spacer in gemini surfactants enhances protein extraction efficiency, indicating their potential for

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Anh

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EN