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Bone marrow stromal cells prepared using AB serum and bFGF for hematopoietic stem cells expansion by Miki Yamaguchi; Fumiya Hirayama; Shinobu Wakamoto; Mitsuhiro Fujihara; Hideaki Murahashi; Norihiro Sato; Kenji Ikebuchi; Ken‐ichi Sawada; Takao Koike; Mikinori Kuwabara; Hiroshi Azuma; Hisami Ikeda is a Medicine article available to read on EtoBox.

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**BACKGROUND**: An ex vivo culture system was previously established for stem cell expansion using human marrow stromal cells and serum‐free medium. However, the stromal cells were prepared using long‐term culture medium containing horse serum and FCS, which may transmit infectious diseases of xenogeneic origin. In this study, therefore, a method was established to prepare stromal cells using an AB serum‐based medium. In the case that serum from a transplant recipient or PBPC donor is available, additional infectious diseases would not be transmitted. **STUDY DESIGN AND METHODS**: Cord blood CD34+ cells were cultured with thrombopoietin, stem cell factor, and flt3/flk2 ligand on a monolayer of human marrow primary stromal cells prepared using long‐term culture medium or AB serum‐based medium. After 2 weeks, clonogenic progenitor activity and SCID mouse‐reconstituting cell activity were assayed. mRNA expression of cytokines and Notch ligand by stromal cells was also examined. **RESULTS**: There were no remarkable differences in expansion‐supporting activity and mRNA expression between stromal cells established by the two methods. **CONCLUSION**: An ex vivo expansion system completel

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Author

Miki Yamaguchi; Fumiya Hirayama; Shinobu Wakamoto; Mitsuhiro Fujihara; Hideaki Murahashi; Norihiro Sato; Kenji Ikebuchi; Ken‐ichi Sawada; Takao Koike; Mikinori Kuwabara; Hiroshi Azuma; Hisami Ikeda

Publisher

Elsevier Science; John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Elsevier; Elsevier BV; Wiley (ISSN 0372-1248)

Published

2002

Language

EN

Field

Medicine (Health Sciences)