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Capillary Electrophoresis in the Analysis of Humic Substances by Ph. Schmitt-Kopplin; A.W. Garrison; E.M. Perdue; D. Freitag; A. Kettrup is a Chemistry article available to read on EtoBox.

What is Capillary Electrophoresis in the Analysis of Humic Substances about?

Humic substances, extracted as mixtures from soil and surface waters according to their solubility in acids and bases, are relatively high-molecular-mass polyelectrolytes containing aromatic, aliphatic and heterocyclic subunits. The degree of ionization of their phenolic and carboxylic groups is governed by the capillary electrophoresis (CE) buffer pH. In CE, fulvic acids exhibit a consistent and characteristic set of sharp peaks (phenolic acids), extending from a humic ''hump'' whose average electrophoretic mobility (AEM) depends on humic structure and buffer composition; humic acids give only the ''hump''. Special attention must be given to the interpretation of CE electropherograms when fingerprinting humic substances with borate buffers because observed peaks do not necessarily indicate distinct humic fractions, but may be artifacts caused by the interaction of borate ions with 1,2-and 1,3-diols present in the humic mixtures. Depending on the molarity of borate ions in the separation buffer, humic acids exhibit electropherograms with sharp peaks extending from the ''humic hump'' and corresponding to borate complexes. The potential of capillary zone electrophoresis for the compa

Who reads Capillary Electrophoresis in the Analysis of Humic Substances?

Author

Ph. Schmitt-Kopplin; A.W. Garrison; E.M. Perdue; D. Freitag; A. Kettrup

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

1998

Language

EN

Field

Chemistry (Physical Sciences)