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Optimized expression and enhanced production of alkaline protease by genetically modified Bacillus licheniformis 2709 by Zhou, Cuixia; Zhou, Huiying; Li, Dengke; Zhang, Huitu; Wang, Hongbin; Lu, Fuping is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background __Bacillus licheniformis__ 2709 is extensively applied as a host for the high-level production of heterologous proteins, but __Bacillus__ cells often possess unfavorable wild-type properties, such as production of viscous materials and foam during fermentation, which seriously influenced the application in industrial fermentation. How to develop it from a soil bacterium to a super-secreting cell factory harboring less undomesticated properties always plays vital role in industrial production. Besides, the optimal expression pattern of the inducible enzymes like alkaline protease has not been optimized by comparing the transcriptional efficiency of different plasmids and genomic integration sites in __B. licheniformis__. ## Result __Bacillus licheniformis__ 2709 was genetically modified by disrupting the native __lchAC__ genes related to foaming and the __eps__ cluster encoding the extracellular mucopolysaccharide via a markerless genome-editing method. We further optimized the expression of the alkaline protease gene (__aprE__) by screening the most efficient expression system among different modular plasmids and genomic loci. The results indicated that genomic expres

Who reads Optimized expression and enhanced production of alkaline protease by genetically modified Bacillus licheniformis 2709?

Author

Zhou, Cuixia; Zhou, Huiying; Li, Dengke; Zhang, Huitu; Wang, Hongbin; Lu, Fuping

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2002-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1475-2859)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)