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DNA replication is not restricted to specific regions in young vegetative Streptomyces mycelia by Christel Kummer; Sigrid Kretschmer; Dr. C. Kummer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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In order to determine the localization of DNA-synthesis in Streptomyces granaticolor and Streptomyces hygrosoopicus, mycelia (growing either on agar or in liquid medium) were pulse-labelled with SH-thymidine and prepared for autoradiography. The distribution of silver grains showed no regions of preferential incorporation of JH-thymidine in mycelia up 300 pm in length. Since mycelia grow by apical elongation of hyphae, the frequency of silver grains was quantitatively analysed along individual main hyphase. No significant difference of labelling was found within zones of different age up to a distance of 80 pm from the hyphal tip. Also, the very youngest part of the hyphae enclosing only the most apically situated nucleoid did not show any deviation from the average frequency of silver grains.

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Author

Christel Kummer; Sigrid Kretschmer; Dr. C. Kummer

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Akademie Verlag; Wiley (ISSN 0233-111X)

Published

1985

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)