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What is Selecting Aptamers for Gold Nanoparticle Assays about?

This document outlines a method for selecting structure-switching aptamers for small molecule targets, specifically using cortisol as a model, to enhance biosensing applications. The proposed protocol utilizes a colorimetric gold nanoparticle assay that provides a rapid and simple detection method, leveraging the conformational changes of aptamers upon target binding. Key modifications to traditional SELEX methods are introduced to improve partitioning efficiency and specificity, making it suitable for smal

Author

Pham Bach

Language

EN