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The document discusses various strategies for manipulating gene expression in prokaryotes, particularly focusing on the T7 RNA polymerase expression system, codon optimization, and addressing challenges such as protein misfolding and metabolic load. It outlines methods to enhance protein stability, secretion, and expression efficiency, including the use of fusion proteins and chromosomal integration. Additionally, it covers approaches to reduce acetate production and improve protein folding in E. coli, ulti
- Author
- nongnapat.pho
- Language
- EN