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Characterization of TRZ1 , a yeast homolog of the human candidate prostate cancer susceptibility gene ELAC2 encoding tRNase Z by Yang Chen; Audrey Beck; Christina Davenport; Yuan Chen; Donna Shattuck; Sean V Tavtigian is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Characterization of TRZ1 , a yeast homolog of the human candidate prostate cancer susceptibility gene ELAC2 encoding tRNase Z about?

## Background In humans, mutation of ELAC2 is associated with an increased risk of prostate cancer. ELAC2 has been shown to have tRNase Z activity and is associated with the γ-tubulin complex. ## Results In this work, we show that the yeast homolog of ELAC2, encoded by __TRZ1__ (tRNase Z 1), is involved genetically in RNA processing. The temperature sensitivity of a __trz1__ mutant can be rescued by multiple copies of __REX2__, which encodes a protein with RNA 3' processing activity, suggesting a role of Trz1p in RNA processing __in vivo__. Trz1p has two putative nucleotide triphosphate-binding motifs (P-loop) and a conserved histidine motif. The histidine motif and the putative nucleotide binding motif at the C-domain are important for Trz1p function because mutant proteins bearing changes to the critical residues in these motifs are unable to rescue deletion of __TRZ1__. The growth defect exhibited by __trz1__ yeast is not complemented by the heterologous ELAC2, suggesting that Trz1p may have additional functions in yeast. ## Conclusion Our results provide genetic evidence that prostate cancer susceptibility gene ELAC2 may be involved in RNA processing, especially rRNA processing

Who reads Characterization of TRZ1 , a yeast homolog of the human candidate prostate cancer susceptibility gene ELAC2 encoding tRNase Z?

Author

Yang Chen; Audrey Beck; Christina Davenport; Yuan Chen; Donna Shattuck; Sean V Tavtigian

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-; Springer Science and Business Media LLC (ISSN 1471-2199)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)