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The document describes a method for quantifying melatonin levels in human saliva using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with stable isotope dilution. Saliva samples were collected from nurses working night shifts and a patient with sleep issues. 7-D-melatonin was used as an internal standard. The limit of detection was 1.05 pg/ml and limit of quantification was 3.0 pg/ml, showing high sensitivity and specificity. The method was used to determine the circadian rhythm of melatonin in
- Author
- Erasmus Cudjoe
- Language
- EN