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Isolation and Structure–Bioactivity Characterization of Glycosylated N-Pro-Opiomelanocortin Isoforms by J. Lu; E. Swinnen; P. Proost; B. De Vreese; H. Vankelecom; C. Denef is a Neuroscience article available to read on EtoBox.

What is Isolation and Structure–Bioactivity Characterization of Glycosylated N-Pro-Opiomelanocortin Isoforms about?

## Abstract The __N__‐terminal fragment of mouse pro‐opiomelanocortin (__N__‐POMC) was isolated from AtT‐20 cell‐conditioned medium on the basis of immunoreactivity to an anti‐POMC1‐50 monoclonal antibody by a concentration step, a cation exchange step, reversed phase high‐performance liquid chromatography (HPLC) and size exclusion HPLC. Two groups of __N__‐POMC isoforms with a molecular weight (MW) of approximately 11 kDa and 13 kDa, respectively, were identified by mass spectrometry and __N__‐terminal amino acid sequencing. __C__‐terminal sequencing indicated that 11 kDa isoforms correspond to POMC1‐74 and 13 kDa isoforms to POMC1‐95. Isoforms from both groups enhanced the prolactin mRNA content (measured by means of TaqMan real‐time reverse transcription‐polymerase chain reaction) in cultured rat pituitary cell aggregates in a dose‐dependent manner, but not all of them showed this activity. POMC1‐74 compounds were significantly more potent than POMC1‐95 isoforms. The observed effects were abolished by coincubation with the monoclonal anti‐POMC1‐50 antibody, showing the specificity of this biological action. Incorporation of bromodeoxyuridine into DNA of immunostained lactotrophs

Who reads Isolation and Structure–Bioactivity Characterization of Glycosylated N-Pro-Opiomelanocortin Isoforms?

Author

J. Lu; E. Swinnen; P. Proost; B. De Vreese; H. Vankelecom; C. Denef

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0953-8194)

Published

2002

Language

EN

Field

Neuroscience (Life Sciences)