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Performance of a Two-Stage Membrane System for Bromelain Separation from Pineapple Waste Mixture as Impacted by Enzymatic Pre-Treatment and Diafiltration by Mohd Zuhair Mohd Nor; Advanced Food Systems Research Unit, College of Health and Biomedicine, Victoria University, PO Box 14428, 8001 Melbourne, Australia; Lata Ramchandran; Mikel Duke; Todor Vasiljevic; Department of Process and Food Engineering, Faculty of Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Parmalat, Lot 4 Third Street, Harvey, Western Australia 6220, Australia; Institute for Sustainability and Innovation, College of Engineering and Science, Victoria University, PO Box 14428, 8001 Melbourne, Australia; Advanced Food Systems Research Unit, College of Health and Biomedicine, Victoria University, PO Box 14428, 8001 Melbourne, Australia is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Performance of a Two-Stage Membrane System for Bromelain Separation from Pineapple Waste Mixture as Impacted by Enzymatic Pre-Treatment and Diafiltration about?

Membrane technology has been successfully applied for the purification of bromelain, a protease enzyme from pineapple. However, the current system operates less optimally in terms of flux and separation primarily due to properties of the feed, such as viscosity. Hence, in this study, enzymatic pretreatment and diafiltration operation were employed in a two-stage ultrafiltration (UF) system to enhance the performance of the purification and concentration process of bromelain enzyme from an extract of pineapple crude waste mixture (CWM). Pretreatment of the CWM extract using either pectinase or cellulase, or the combination of both, was applied and compared regarding the apparent viscosity reduction. Diafiltration step was introduced in UF stage 2 and observations on the flux performance, enzyme recovery and enzyme purity were made. A 12% apparent viscosity reduction was achieved when the CWM extract was pretreated with pectinase which led to 37-38% improvement in the flux performance of both UF stages, as well as higher enzyme recovery in UF stage 1. The introduction of diafiltration mode in UF stage 2 managed to sustain high flux values while yielding 4.4-fold enzyme purity (higher

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Author

Mohd Zuhair Mohd Nor; Advanced Food Systems Research Unit, College of Health and Biomedicine, Victoria University, PO Box 14428, 8001 Melbourne, Australia; Lata Ramchandran; Mikel Duke; Todor Vasiljevic; Department of Process and Food Engineering, Faculty of Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Parmalat, Lot 4 Third Street, Harvey, Western Australia 6220, Australia; Institute for Sustainability and Innovation, College of Engineering and Science, Victoria University, PO Box 14428, 8001 Melbourne, Australia; Advanced Food Systems Research Unit, College of Health and Biomedicine, Victoria University, PO Box 14428, 8001 Melbourne, Australia

Publisher

Faculty of Food Technology and Biotechnology - University of Zagreb

Published

2017

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)