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GENETIC VARIABILITY AND MOLECULAR PHYLOGENY OF DINOPHYSIS SPECIES (DINOPHYCEAE) FROM NORWEGIAN WATERS INFERRED FROM SINGLE CELL ANALYSES OF rDNA^1^ by Bente Edvardsen; Kamran Shalchian‐Tabrizi; Kjetill S. Jakobsen; Linda K. Medlin; Einar Dahl; Sissel Brubak; Eystein Paasche is a Earth and Planetary Sciences article available to read on EtoBox.
What is GENETIC VARIABILITY AND MOLECULAR PHYLOGENY OF DINOPHYSIS SPECIES (DINOPHYCEAE) FROM NORWEGIAN WATERS INFERRED FROM SINGLE CELL ANALYSES OF rDNA^1^ about?
The objectives of this study were to determine rDNA sequences of the most common __Dinophysis__ species in Scandinavian waters and to resolve their phylogenetic relationships within the genus and to other dinoflagellates. A third aim was to examine the intraspecific variation in __D. acuminata__ and __D. norvegica__, because these two species are highly variable in both morphology and toxicity. We obtained nucleotide sequences of coding (small subunit [SSU], partial large subunit [LSU], 5.8S) and noncoding (internal transcribed spacer [ITS]1, ITS2) parts of the rRNA operon by PCR amplification of one or two __Dinophysis__ cells isolated from natural water samples. The three photosynthetic species __D. acuminata__, __D. acuta__, and __D. norvegica__ differed in only 5 to 8 of 1802 base pairs (bp) within the SSU rRNA gene. The nonphotosynthetic __D. rotundata__ (synonym __Phalacroma rotundatum__[Claparède et Lachmann] Kofoid et Michener), however, differed in approximately 55 bp compared with the three photosynthetic species. In the D1 and D2 domains of LSU rDNA, the phototrophic species differed among themselves by 3 to 12 of 733 bp, whereas they differed from __D. rotundata__ by mo
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It is typically read by researchers, students, and practitioners in Earth and Planetary Sciences.
- Author
- Bente Edvardsen; Kamran Shalchian‐Tabrizi; Kjetill S. Jakobsen; Linda K. Medlin; Einar Dahl; Sissel Brubak; Eystein Paasche
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3646)
- Published
- 2003
- Language
- EN
- Field
- Earth and Planetary Sciences (Physical Sciences)