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A Post-preservation Vascular Flush Removes Significant Populations of Donor Leukocytes Prior to Lung Transplantation by Alexandra L. Ball; Rebecca J. Edge; Kavit Amin; William R. Critchley; Gareth J. Howell; Nizar Yonan; John P. Stone; James E. Fildes is a Medicine article available to read on EtoBox.

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## Background: Donor leukocytes are intrinsically involved in acute lung allograft rejection, via self-presentation of donor antigens to recipient leukocytes. therapeutic modalities to remove donor leukocytes are currently unavailable. we evaluated if a vascular flush immediately following preservation can be used for this purpose. ## Methods: A post-preservation flush was performed with steen solution in n = 6 porcine lungs following static cold storage. the first 500 ml effluent from the left atrium was collected and an inflammatory profile performed. ## Results: A total of 1.17 billion (±2.8 × 10) viable leukocytes were identified within the effluent. t cells were the dominant cell population, representing 82% of the total mobilised leukocytes, of which <0.01% were regulatory t cells. il-18 was the most abundant cytokine, with a mean concentration of 84,216 pg (±153,552 pg). in addition, there was a mean concentration of 8819 ng (±4415) cell-free mitochondrial dna. ## Conclusion: There is an immediate transfer of donor leukocytes, cytokines and damage-associated molecular patterns following reperfusion. such a pro-inflammatory donor load may enhance alloantigen presentation and

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Author

Alexandra L. Ball; Rebecca J. Edge; Kavit Amin; William R. Critchley; Gareth J. Howell; Nizar Yonan; John P. Stone; James E. Fildes

Publisher

Elsevier BV

Published

2020

Language

EN

Field

Medicine (Health Sciences)