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Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance by Ming-Wei Li; Rui-Qing Lin; Hui-Qun Song; Rehana A. Sani; Xiang-Yun Wu; Xing-Quan Zhu is a Engineering article available to read on EtoBox.

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## Abstract Sequence variability in three mitochondrial DNA (mtDNA) regions, namely cytochrome __c__ oxidase subunit 1 (__cox__1), NADH dehydrogenase subunits 1 and 4 (__nad__1 and __nad__4), among and within __Toxocara canis__, __T. cati__, __T. malaysiensis__, __T. vitulorum__ and __Toxascaris leonina__ from different geographical origins was examined by a mutation‐scanning approach. A portion of the __cox__1 gene (p__cox__1), a portion of the __nad__1 and __nad__4 genes (p__nad__1 and p__nad__4) were amplified separately from individual ascaridoid nematodes by polymerase chain reaction and the amplicons analyzed by single‐strand conformation polymorphism (SSCP). Representative samples displaying sequence variation in SSCP profiles were subjected to sequencing in order to define genetic markers for their specific identification and differentiation. While the intra‐specific sequence variations within each of the five ascaridoid species were 0.2–3.7% for p__cox__1, 0–2.8% for p__nad__1 and 0–2.3% for p__nad__4, the inter‐specific sequence differences were significantly higher, being 7.9–12.9% for p__cox__1, 10.7–21.1% for p__nad__1 and 12.9–21.7% for p__nad__4, respectively. Phylog

Who reads Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance?

Author

Ming-Wei Li; Rui-Qing Lin; Hui-Qun Song; Rehana A. Sani; Xiang-Yun Wu; Xing-Quan Zhu

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0173-0835)

Published

2008

Language

EN

Field

Engineering (Physical Sciences)