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What is Acid Fast Stain Procedure and Analysis about?

The Acid fast stain is used to detect Mycobacterium, which contains mycolic acids in its cell wall that confer acid-fast properties. The stain uses carbol fuchsin dye, which is retained in acid-fast bacteria after decolorization with acid alcohol. Mycobacterium will appear pink, while non-acid fast bacteria like Staphylococcus aureus will appear blue. Mycolic acids allow Mycobacterium like M. tuberculosis and M. leprae to survive in macrophages and resist disinfectants, contributing to their virulence and t

Author
Martina Micic
Language
EN

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