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Characterization of antibacterial activity of a N-acetylmuramoyl-l-alanine amidase produced by Latilactobacillus sakei isolated from salami by Adriana Lopez-Arvizu; Diana Rocha-Mendoza; Edith Ponce-Alquicira; Israel García-Cano is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Characterization of antibacterial activity of a N-acetylmuramoyl-l-alanine amidase produced by Latilactobacillus sakei isolated from salami about?

Lactic acid bacteria are the predominant group within meat products, whose metabolites such as bacteriocins and peptidoglycan hydrolases inhibit pathogenic or spoilage bacteria. Fermented meat products, as a salami, is a good source to analyze the viable microbiota, due to these products present a low risk to consumer health. The aim of this work was to identify the lactic acid bacteria with broad antibacterial activity present in salami, purify the protein responsible for this activity, achieve antagonistic spectrum and perform the biochemical characterization. Five strains from salami were selected, isolated and identified by 16S rRNA gene sequencing. The antimicrobial activity was evaluated by antagonism assay and zymography, using spoilage microorganisms commonly found in meat products. The strain that showed a broad antibacterial activity was Latilactobacillus sakei and the antibacterial activity was given by a protein with 75-kDa of molecular mass, identified by LC/MALDI-TOF/TOF. The sequence analysis showed 67% of identity with a N-acetylmuramoyl-l-alanine amidase protein with five non-identical LysM domains. The purified protein showed an optimal pH of 8.0 and heat resistan

Who reads Characterization of antibacterial activity of a N-acetylmuramoyl-l-alanine amidase produced by Latilactobacillus sakei isolated from salami?

Author

Adriana Lopez-Arvizu; Diana Rocha-Mendoza; Edith Ponce-Alquicira; Israel García-Cano

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)