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Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex by cryo-electron microscopy single particle analysis by Gaoxingyu Huang; Yanqing Zhang; Xuechen Zhu; Chao Zeng; Qifan Wang; Qiang Zhou; Qinghua Tao; Minhao Liu; Jianlin Lei; Chuangye Yan; Yigong Shi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex by cryo-electron microscopy single particle analysis about?
The nuclear pore complex (NPC) exhibits structural plasticity and has only been characterized at local resolutions of up to 15 Å for the cytoplasmic ring (CR). Here we present a single-particle cryo-electron microscopy (cryo-EM) structure of the CR from __Xenopus laevis__ NPC at average resolutions of 5.5–7.9 Å, with local resolutions reaching 4.5 Å. Improved resolutions allow identification and placement of secondary structural elements in the majority of the CR components. The two Y complexes in each CR subunit interact with each other and associate with those from flanking subunits, forming a circular scaffold. Within each CR subunit, the Nup358-containing region wraps around the stems of both Y complexes, likely stabilizing the scaffold. Nup205 connects the short arms of the two Y complexes and associates with the stem of a neighboring Y complex. The Nup214-containing region uses an extended coiled-coil to link Nup85 of the two Y complexes and protrudes into the axial pore of the NPC. These previously uncharacterized structural features reveal insights into NPC assembly.
Who reads Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex by cryo-electron microscopy single particle analysis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Gaoxingyu Huang; Yanqing Zhang; Xuechen Zhu; Chao Zeng; Qifan Wang; Qiang Zhou; Qinghua Tao; Minhao Liu; Jianlin Lei; Chuangye Yan; Yigong Shi
- Publisher
- Nature Publishing Group; Springer Science and Business Media LLC (ISSN 0007-0610)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)