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Plasmid transfer and expression of the transfer (tra) gene product of plasmid pIJ101 are temporally regulated during the Streptomyces lividans life cycle by Gregg S. Pettis; Stanley N. Cohen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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We previously have shown that a chromosomally integrated copy of the __tra__ gene of plasmid pIJ101 under the control of the KorA repressor protein, which regulates transcription of __tra__ as well as its own synthesis, can promote the conjugal transfer of both chromosomal and plasmid genes in __Streptomyces lividans__. Using an antibody generated against a fusion protein containing the C‐terminal portion of Tra, we show here that this essential conjugation protein is present in membrane fractions of both surface‐grown __S. lividans__, which mate readily, and of cells grown in liquid culture, where mating has not been found. Expression of Tra during the __S. lividans__ life cycle was temporally regulated and was reduced late during vegetative growth so that little or no Tra protein was detected in cells as they began to differentiate morphologically and produce secondary metabolites. Comparison of the membrane concentration of Tra protein with __tra__ mRNA concentration during the __S. lividans__ life cycle indicated that the disappearance of Tra is post‐transcriptionally controlled and thus is not mediated by KorA. The results of ‘interrupted mating’ experiments, together with the

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Author

Gregg S. Pettis; Stanley N. Cohen

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)