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Metal-ion transporter SLC39A8 is required for brain manganese uptake and accumulation 1 Results 2 Inactivation of Slc39a8 reduces Mn accumulation in the liver, spleen, lung, and brain of Slc39a14 KO; Slc39a8 iKO mice 2 Neonatal inactivation of Slc39a8 in Slc39a14 KO mice prevents Mn accumulation in the brain 4 Slc39a8 iKO mice display impaired 54Mn uptake by the brain and spleen 4 Slc39a8 inactivation is efficient in brain microvessels but not choroid plexus 4 Discussion 6 Experimental procedures 9 Animals and diets 9 RNA isolation and determination of mRNA levels 10 Metal measurements 10 Tissue 54Mn accumulation in vivo 10 Brain microvessel enrichment 10 Western blot analysis 10 Statistical analysis 11 Data availability 11 Supporting information 11 Author contributions 11 Funding and additional information 11 References 11
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