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Systematic analysis of the ABO gene diversity within exons 6 and 7 by PCR screening reveals new ABO alleles by Axel Seltsam; Michael Hallensleben; Anke Kollmann; Jürgen Burkhart; Rainer Blasczyk is a Medicine article available to read on EtoBox.
What is Systematic analysis of the ABO gene diversity within exons 6 and 7 by PCR screening reveals new ABO alleles about?
**BACKGROUND**: Mutations critical for ABO blood group phenotypes have predominantly been found in exons 6 and 7 of the __ABO__ gene, both of which encode the catalytic domain of ABO glycosyltransferase. To design rapid and reliable __ABO__ genotyping assays, a profound knowledge of the prevalent alleles is required and a reliable sequence database needs to be established. **STUDY DESIGN AND METHODS**: A PCR screening system was established consisting of 102 different PCRs, each specific for a single nucleotide (nt) variation. The primer mixes were developed to walk from the 5′ to the 3′ end of exons 6 and 7 of the __ABO__ gene to screen for nt mutations at 50 known polymorphic sites. A total of 109 unrelated individuals with common and rare ABO characteristics were screened. All blood samples in which the PCR results were inconclusive or inconsistent with the ABO phenotypes were subjected to sequence analysis of exons 6 and 7. **RESULTS**: The results of PCR screening were conclusive and consistent with the ABO phenotypes in 90 cases. In the remaining 19 cases, PCR screening revealed unusual allele combinations or amplification results that were incompatible with known __ABO__ all
Who reads Systematic analysis of the ABO gene diversity within exons 6 and 7 by PCR screening reveals new ABO alleles?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Axel Seltsam; Michael Hallensleben; Anke Kollmann; Jürgen Burkhart; Rainer Blasczyk
- Publisher
- Elsevier Science; John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Elsevier; Elsevier BV; Wiley (ISSN 0372-1248)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)