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Fic domain-catalyzed adenylylation: Insight provided by the structural analysis of the type IV secretion system effector BepA by Dinesh V. Palanivelu; Arnaud Goepfert; Marcel Meury; Patrick Guye; Christoph Dehio; Tilman Schirmer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Fic domain-catalyzed adenylylation: Insight provided by the structural analysis of the type IV secretion system effector BepA about?

## Abstract Numerous bacterial pathogens subvert cellular functions of eukaryotic host cells by the injection of effector proteins via dedicated secretion systems. The type IV secretion system (T4SS) effector protein BepA from __Bartonella henselae__ is composed of an N‐terminal Fic domain and a C‐terminal __Bartonella__ intracellular delivery domain, the latter being responsible for T4SS‐mediated translocation into host cells. A proteolysis resistant fragment (residues 10–302) that includes the Fic domain shows autoadenylylation activity and adenylyl transfer onto Hela cell extract proteins as demonstrated by autoradiography on incubation with α‐[^32^P]‐ATP. Its crystal structure, determined to 2.9‐Å resolution by the SeMet‐SAD method, exhibits the canonical Fic fold including the HPFxxGNGRxxR signature motif with several elaborations in loop regions and an additional β‐rich domain at the C‐terminus. On crystal soaking with ATP/Mg^2+^, additional electron density indicated the presence of a PP~i~/Mg^2+^ moiety, the side product of the adenylylation reaction, in the anion binding nest of the signature motif. On the basis of this information and that of the recent structure of IbpA(

Who reads Fic domain-catalyzed adenylylation: Insight provided by the structural analysis of the type IV secretion system effector BepA?

Author

Dinesh V. Palanivelu; Arnaud Goepfert; Marcel Meury; Patrick Guye; Christoph Dehio; Tilman Schirmer

Publisher

Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)