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Pulsed-amperometric Detection of Urea in Blood Samples on a Conducting Polypyrrole-urease Biosensor by S.B. Adeloju; S.J. Shaw; G.G. Wallace is a Chemistry article available to read on EtoBox.
What is Pulsed-amperometric Detection of Urea in Blood Samples on a Conducting Polypyrrole-urease Biosensor about?
A rapid and highly sensitive method is described for reliable flow injection analysis of urea based on pulsed-amperometric detection with a polypyrrole-urease biosensor. The optimum conditions were 0.05 M phosphate buffer (pH 7.0) a negative potential pulse between -70 mV (El) and -400 mV (Ez) vs Ag/AgCl, a pulse width of 120 mS, and a flow rate of 0.1 ml min-'. A detection limit (3a) of 60 ug 1-l of urea was achieved by this method and the response was linear in the range 100-4500 ug 1-l. The biosensor was very stable in the flow system, even after two weeks of continuous use. Excellent recovery of urea (95%-l 12%) was accomplished employing this method in simulated samples. Successful application of the method to the determination of urea in blood plasma is reported.
Who reads Pulsed-amperometric Detection of Urea in Blood Samples on a Conducting Polypyrrole-urease Biosensor?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- S.B. Adeloju; S.J. Shaw; G.G. Wallace
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 1997
- Language
- EN
- Field
- Chemistry (Physical Sciences)