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What is Purification of GALLS Protein for Gene Transfer about?

The document summarizes research on purifying the GALLS protein from Agrobacterium rhizogenes, which is required for gene transfer to plants. Key points: - A mutation was introduced to the GALLS gene to abolish translation of the GALLS-CT protein and add a histidine tag for purification of the full-length GALLS-FL protein. - The mutated GALLS gene was inserted into a plasmid and transformed into Agrobacterium cells. - Initial results showed the mutation was incorporated correctly and GALLS-FL protein c

Author

Srikant Mishra

Language

EN