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Can I read Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom on EtoBox?
Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom by Anwar Ullah; Priscila Oliveira de Giuseppe; Mario Tyago Murakami; Dilza Trevisan-Silva; Ana Carolina Martins Wille; Daniele Chaves-Moreira; Luiza Helena Gremski; Rafael Bertoni da Silveira; Andrea Sennf-Ribeiro; Olga Meiri Chaim; Silvio Sanches Veiga; Raghuvir Krishnaswamy Arni is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom about?
Phospholipases D are the major dermonecrotic component of Loxosceles venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses. Phospholipases D can be classified into two classes depending on their catalytic efficiency and the presence of an additional disulfide bridge. In this work, both wild-type and H12A-mutant forms of the class II phospholipase D from L. intermedia venom were crystallized. Wild-type and H12A-mutant crystals were grown under very similar conditions using PEG 200 as a precipitant and belonged to space group P12(1)1, with unit-cell parameters a = 50.1, b = 49.5, c = 56.5 Å, β = 105.9°. Wild-type and H12A-mutant crystals diffracted to maximum resolutions of 1.95 and 1.60 Å, respectively.
Who reads Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Anwar Ullah; Priscila Oliveira de Giuseppe; Mario Tyago Murakami; Dilza Trevisan-Silva; Ana Carolina Martins Wille; Daniele Chaves-Moreira; Luiza Helena Gremski; Rafael Bertoni da Silveira; Andrea Sennf-Ribeiro; Olga Meiri Chaim; Silvio Sanches Veiga; Raghuvir Krishnaswamy Arni
- Publisher
- John Wiley and Sons; International Union of Crystallography; John Wiley and Sons Ltd; International Union of Crystallography (IUCr) (ISSN 1744-3091)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)