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Can I read Activation of Transcription as a General Mechanism of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Action on EtoBox?

Activation of Transcription as a General Mechanism of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Action by Joan M. Fisher; Keith W. Jones; James P. Whitlock Jr. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Activation of Transcription as a General Mechanism of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Action about?

We studied the response t o 2,3,7,8-tetrachlorodibenzo-pdioxin (TCDD) of mouse hepatoma cells that contain a single, integrated copy o f a chimeric gene under the control of a dioxin-responsive DNA domain, which was originally associated with the cytochrome P450/A 7 gene. Our findings indicate that TCDD increases the RNA polymerase Il-catalyzed transcription rate of the chimeric gene and that the transcripts are initiated at the correct promoter. Therefore, the dioxin-responsive DNA operates as a bona fide transcriptional enhancer. Other studies imply that the Ah receptor mediates the transcriptional response t o TCDD. Our results indicate that the Ah receptor-dependent, dioxin-responsive enhancer can activate transcription when in a regulatory context and in a chromosomal location different from those o f the cytochrome P4501AI gene. Therefore, in principle, the receptor-enhancer system represents a mechanism by which numerous genes can respond to aromatic hydrocarbons in the environment.

Who reads Activation of Transcription as a General Mechanism of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Action?

It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.

Author
Joan M. Fisher; Keith W. Jones; James P. Whitlock Jr.
Publisher
John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)
Published
1989
Language
EN
Field
Biochemistry, Genetics and Molecular Biology (Life Sciences)