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Effect of Epidermal Growth Factor on Preimplantation Development and Its Receptor Expression in Porcine Embryos\* by Zhixin Wei; Kwang‐Wook Park; Bill N. Day; Randall S. Prather is a Medicine article available to read on EtoBox.
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## Abstract The present study aimed to determine the influence of exogenous epidermal growth factor (EGF) on in vitro preimplantation porcine embryo development and its mRNA expression for EGF receptor (EGFR). Oocytes were aspirated from abattoir ovaries, selected and cultured in defined, protein‐free media for 44 hr before in vitro fertilization (IVF). Thirty‐six hours after IVF, two‐cell stage embryos were selected and treated or cultured until embryo treatment. In experiment 1, compact morulae were selected on day 4 after IVF and randomly allocated into 5 groups: NCSU 23 with PVA as group 1; NCSU 23 with PVA and 0.1 ng/ml, 1.0 ng/ml, 10.0 ng/ml EGF as group 2, 3, 4, respectively; NSCU 23 with 0.4% BSA as group 5. In experiment 2, treatment groups were the same as in experiment 1 except that 0.1% crystallized BSA was added to both washing media and all treatment groups instead of PVA. In experiments 3 and 4, two‐cell stage embryos were treated and cultured in the same experimental design as experiments 1 and 2, respectively. RT‐PCR was used to detect the mRNA expression of EGF receptor in compact morulae and blastocysts. The PCR products were subjected to direct DNA sequencing. T
Who reads Effect of Epidermal Growth Factor on Preimplantation Development and Its Receptor Expression in Porcine Embryos\*?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Zhixin Wei; Kwang‐Wook Park; Bill N. Day; Randall S. Prather
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1040-452X)
- Published
- 2001
- Language
- EN
- Field
- Medicine (Life Sciences)