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LDH Kinetic Assay Protocol by Staci Washington is a document available to read on EtoBox.
What is LDH Kinetic Assay Protocol about?
This document provides instructions for using a lactate dehydrogenase (LDH) kinetic assay kit, including storage and handling of reagents, specimen collection and storage, assay procedures, calculations, quality control, and expected reference values. The assay uses LDH to catalyze the conversion of pyruvate and NADH to lactate and NAD, and measures the absorbance change at 340nm to determine LDH activity levels. Reference values for LDH at different temperatures are provided.
- Author
- Staci Washington
- Language
- EN