Can I read E. coli Plasmid Vectors Volume 235 on EtoBox?

E. coli Plasmid Vectors Volume 235 by Casali, Nicola; Preston, Andrew is a scholarly article available to read on EtoBox.

What is E. coli Plasmid Vectors Volume 235 about?

Manipulation of recombinant DNA, which is almost exclusively performed using the host E. coli, constitutes one of the fundamental methodologies of molecular biotechnology. In E. coli Plasmid Vectors, experienced bench researchers describe their proven techniques for the manipulation of recombinant plasmids utilizing this popular bacterial host. The authors describe readily reproducible methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Also presented are methods for common downstream applications, such as mutagenesis, expression of recombinant proteins and RNA transcripts, and uses of reporter genes. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, tips on troubleshooting and avoiding known pitfalls and, where needed, a discussion

Author

Casali, Nicola; Preston, Andrew

Publisher

Humana Press : Imprint: Humana

Published

2003

Language

EN

ISBN

9781280842825