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Can I read HPLC–MS/MS Analysis and Study on the Adsorption/Desorption Characteristics of Ginsenosides on Anion-Exchange Macroporous Resins on EtoBox?
HPLC–MS/MS Analysis and Study on the Adsorption/Desorption Characteristics of Ginsenosides on Anion-Exchange Macroporous Resins by Siming Wang; Rui Zou; Fajie Wu; Shuai Bian; Yu Xin; Tianzhu He; Weinan Wang; Zhidong Qiu is a Chemistry article available to read on EtoBox.
What is HPLC–MS/MS Analysis and Study on the Adsorption/Desorption Characteristics of Ginsenosides on Anion-Exchange Macroporous Resins about?
Anion exchange resin is a modified version of polar/nonpolar macro porous resins with enhanced selectivity on compounds carrying negative charges. Although it is a promising adsorbent for the purification of natural products, no research regarding its application on ginsenosides has been reported elsewhere. In this paper, we first isolated total ginsenosides from Panax ginseng and investigated their static adsorption/desorption capacity on different resins, among which D301 resin was selected as the optimum one. A pH value of 8 was chosen for the balanced interaction between total ginsenosides and the adsorbents. Adsorption kinetics and isotherms of total ginsenosides on D301 and D101 resins were investigated to differentiate the adsorption characteristics of anion exchange and nonpolar macroporous resins. A D301-based chromatographic method was established to purify total ginsenosides with settings as follows: sample loading and elution speed = 4 bed volume per hour, breakthrough volume = 2 bed volume, elution solvent = 80% aqueous ethanol, elution volume = 8 bed volume. HPLC-QqQ-MS qualitatively and quantitatively analyzed nine individual ginsenosides from the eluates, demonstrat
Who reads HPLC–MS/MS Analysis and Study on the Adsorption/Desorption Characteristics of Ginsenosides on Anion-Exchange Macroporous Resins?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Siming Wang; Rui Zou; Fajie Wu; Shuai Bian; Yu Xin; Tianzhu He; Weinan Wang; Zhidong Qiu
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Chemistry (Physical Sciences)