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This document details a research article published in the journal Bioanalysis, focusing on validated GC-MS methods for detecting ethylene glycol, diethylene glycol, and triethylene glycol in human serum, plasma, and urine samples. The study addresses safety concerns regarding the use of Polyethylene Glycol 3350 (PEG 3350) in children, as it may break down into potentially harmful substances. The developed methods demonstrated sensitivity and accuracy, with findings indicating low levels of these glycols in

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