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Visual Representation by Atomic Force Microscopy (AFM) of Tomato Spotted Wilt Virus Ribonucleoproteins by Jan-Wolfhard Kellmann; Peter Liebisch; Klaus-Peter Schmitz; Birgit Piechulla is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Atomic force microscopy (AFM) allows the observation of biological material without fixation procedures. Here we present AFM images of ribonucleoproteins (nucleocapsids) derived from a plant infecting RNA virus (tomato spotted wilt virus, TSWV), which have been recorded in contact mode. The nucleocapsids, prepared from systemically infected leaves of tobacco, were spreaded on a glass surface and dried in air, and appeared as regularly formed rings, resembling the proposed pseudocircular and panhandle structure of encapsidated genomic RNA. Average values between 1300 and 2200 nm of nucleocapsid lengths could be related to dimensions estimated by electron microscopy, thereby validating a filamentous configuration of the TSWV ribonucleoproteins. However, to our knowledge regular, ring-like forms of ribonucleoproteins have not been obtained by electron microscopy, which rather showed an amorphous structure of the virus particles. Hence, the AFM approach provides a starting point for further detailed studies on TSWV ribonucleoprotein complexes.

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Author

Jan-Wolfhard Kellmann; Peter Liebisch; Klaus-Peter Schmitz; Birgit Piechulla

Publisher

Walter de Gruyter GmbH

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)