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Increased Midkine Expression in Intrahepatic Cholangiocarcinoma: Immunohistochemical and in Situ Hybridization Analyses by Masako Kato; Takao Shinozawa; Shinsuke Kato; Kanenori Endo; Tadashi Terada is a Medicine article available to read on EtoBox.

What is Increased Midkine Expression in Intrahepatic Cholangiocarcinoma: Immunohistochemical and in Situ Hybridization Analyses about?

**Abstract:** __Aims/Background:__ Midkine (MK) is a novel heparin‐binding growth factor whose gene was identified in embryonal carcinoma cells in the early stages of retinoic acid‐induced differentiation. This study investigates the overexpression of MK in intrahepatic cholangiocarcinoma (CC). __Methods:__ Forty‐five primary CC specimens from patients (aged 19–81 years, 24 males and 21 females) were examined. Histologically, 17 cases of CC were classified as the well‐differentiated type, 19 as moderately‐differentiated and 9 as poorly‐differentiated. Immunohistochemical analysis was performed using a rat IgG~2a~ monoclonal antibody against the carboxyl terminal region of human MK. __Results:__ We successfully applied this monoclonal antibody against MK to analyze archival paraffin sections. The cancer tissues showed a positive reaction to this antibody, and there was an intense reaction in their cytoplasm. Approximately 40% of individuals with CC (17/45) had tumor cells that expressed MK, and these were classified into the following types: moderately‐differentiated type (9/19), well‐differentiated type (8/17) and poorly‐differentiated type (0/9). __In situ__ hybridization analysis

Who reads Increased Midkine Expression in Intrahepatic Cholangiocarcinoma: Immunohistochemical and in Situ Hybridization Analyses?

Author

Masako Kato; Takao Shinozawa; Shinsuke Kato; Kanenori Endo; Tadashi Terada

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley; Research Square (ISSN 0106-9543)

Published

2000

Language

EN

Field

Medicine (Health Sciences)